Histology
Chapter 1- H&E staining
1. The embedding medium for microscopy is
a. paraffin
b. ethyl alcohol
c. plastic material
d. xylol
e. celloidin
2. In H.E. stain sections, the cytoplasm is stained pink by _______, the nucleus is stained purple-blue by________.
a. hematoxylin, Eosin
b Eosin, hematoxylin
c. xylol, Eosin
d. eosin, xylol
3. The procedure of preparation of histologic slides includes mainly________,_________,___________,_________and mounting.
a. fixation, dehydration, embedding, stainin
b. dehydration, fixation, embedding, stainin
c. embedding, fixation, stainin, dehydration
d. fixation, dehydration, stainin, embedding
4. hematoxylin is an ____ dye?
a. acidic
b. basic
c. neutral
both acidic and basic
5. what is the purpose of embedding?
a. to get rid of alcohol
b. mount sections on glass slides
c. obtain the specimen
d. preserve the structural organization of the tissue
6. what is the color of nucleus in H&E staining ?
a. blue
b. red
c. eosinophilic
d. pink
7. how thick microtome should cut the tissue for H&E staining?
a. 1-2 um
b. 0.1-1 um
c. 3-8 um
d. 1 um